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Mouse and human SIRP alpha -CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells
NYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGRWRGGDVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTIEGMIKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNIPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI
Select a 24-well plate or a 48-well plate for cell culture and treatment
② Preparation of color developing solution: The color developing solution A Chromogenic Solution B Mix in equal volumes
Analyze by Pulldown or other Biotin quantitative kit
Alexa Fluor® 647 Mouse Anti-Human CD21 Antibody (S-764-18) Size:100T Mouse and human SIRP alphaProduct Specification Host Mouse Antigen CD21 Synonyms Complement receptor type 2, Cr2, Complement C3d receptor, Epstein Barr virus receptor (EBV receptor) Immunogen Recombinant Protein Location Cell membrane Accession P20023 Clone Number S 764 18 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Purification Protein G Concentration 0. 02mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 0. 1% BSA, 0.